We found a cluster of RBP genes including igf2bp3, zar1, elavl1, rbm25b and related regulatory factors including yy1, sirt1, anp32e, btg4 as novel biomarkers of egg quality. Finally, gene expression was validated by qRT-PCR. The RBPs were more related to egg quality from the PLS-DA analysis. ![]() ![]() Gene function analysis indicated that most of these genes were enriched in RNA-regulated pathways including RNA processing. We profiled the differentially expressed genes and identified a group of RNA-binding proteins (RBPs) as potential regulators. Then RNA-seq was performed for the originally preserved eggs from the low-quality group and high-quality group. Due to the distinct difference in fertilization, hatching and embryo malformation rate between low-quality eggs and high-quality eggs, these two groups were considered for the identification of quality markers. The egg quality was determined by the fertilization rates, hatching rate and embryo malformation rate and divided into three categories, low-quality ( 75%). ![]() ResultsĮggs from 25 female fish were fertilized with sperm from the same fish. In this study, we aim to identify quality instructors from the combination study of fertilization rate, hatching rate, embryo malformation rate and gene expression profile. An effective evaluation of egg quality prior to fertilization is helpful in improving the fertilization rate and survival rate of the larva. Egg quality is a major concern in fish reproduction and development.
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